RocketScript is able to perform reverse transcription reactions throughout a wide range of temperatures from 42°C to 70°C.
RocketScript has enhanced performance to handle high and low input RNA concentrations as well as short and long RT target sizes.
The product contains the enzyme itself, plus RNase inhibitors and all other components necessary for the best reverse transcription results in the tube.
Both CTRT and first-strand cDNA synthesis are both possible, with reaction temperatures ranging from 42°C to 70°C.
Stable at room temperature for a month one year at 4°C and for 2 years in a -20°C freezer
AccuPower RocketScript Cycle RT PreMix is a ready-to-use lyophilized mastermix containing all components for first-strand cDNA synthesis from purified Poly(A) or total RNA template. The premix contains Bioneer's exclusive M-MLV based thermostable reverse transcriptase (RTase), RocketScript, and oligo dT20 for added convenience. Conditions are optimized for Bioneer's patented Cyclic Temperature Reverse Transcription (CTRT) in a premix form. Native M-MLV RTase has maximum activity at relatively low temperatures (42°C), causing several problems in reverse transcription of RNA molecules with complex secondary structure. AccuPower RocketScript Cycle RT PreMix has thermostable activity across a wide temperature range, (42°C - 70°C), allowing efficient cDNA synthesis from virtually any RNA. The lyophilized PreMix contains all components necessary for a successful CTRT reaction, including RTase, RNase inhibitor and buffer components. Just add template RNA, primers and water, and the RT reaction is ready to go.
The authorised South African distributor of this product is under no obligation to honour the manufacture's guarantees/warranties or to provide after-sales service.
Please note that this item is imported from the USA, and is designed to be used in the USA. In addition, if the unit is powered it will come with a US plug and an adapter/transformer may be required. Please click here for more information on power requirements, or check with us if you are unsure or need any assistance!
Please also note that certain items cannot be imported, these include Alcohol, Animals, Batteries, Flammable Materials, Currency, Food, Furs, Chemicals, Explosives, Medications, Plants, Seeds, Supplements, Pressurized Cans, Tactical Equipment, Vitamins, Weaponry and Weaponry Accessories. In these cases, the item and information is displayed for reference purposes only. If you are not sure if we are permitted to bring an item, please send us an e-mail with a link to the item to confirm.
Please also ensure that you are ordering the correct item for your particular application as returns to the USA are costly. Product reviews are also provided for most of our items, which can give you a good idea for possible things to look out for and the quality of the item. By clicking Add to Cart, you are confirming that the item is correct and you accept the conditions listed here.