The PCR Mycoplasma Detection Kit employs a nucleic acid amplification test on the basis of a polymerase chain reaction (PCR). The PCR method allows a fast and highly sensitive detection of mycoplasma contamination in biological samples. The contained primers are specific to a segment of the 16S rRNA region of the mycoplasma genome. The amplified PCR product has a size of approximately 270bp and can directly be made visible in an agarose gel. The selected template is highly conserved within the s. The PCR procedure for mycoplasma detection is well documented in literature and the test is accepted as very specific and sensitive (with the appropriate primers). The primers were tested for years in comparison to the culture detection method and found to be very reliable and specific to Mycoplasma (do not react with mammalian or bacterial cells). The primers have broad detection range - more than 95% of the mycoplasma species found in cell culture.
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