Edvotek 300 Blue/White Cloning of a DNA Fragment and Assay of

Edvotek 300 Blue/White Cloning of a DNA Fragment and Assay of -galactosidase, For 5 Lab Groups
- For 5 Lab Groups; ligation 70 min, transformation 60 min, plating 5 min, incubation overnight transformation 15 min
- Kit includes: instructions, Linearized pUC plasmid and DNA fragment, T4 Ligase, Bacterial LyphoCells for transformation, reconstitution buffer, XGal in solvent, IPTG,
- Kit includes: calcium chloride, antibiotic, ReadyPour Luria Broth Agar, Luria broth media for recovery, growth media, assay components, plastic supplies
- You need: incubation oven, two waterbaths, shaking incubator or shaking waterbath, microwave or hot plate, automatic micropipet and tips
- You need: spectrophotometer, balance, centrifuge, microcentrifuge, glassware and cuvettes, distilled water, ice
When DNA is subcloned in the pUC polylinker region, ï¿½-galactosidase production is interrupted, resulting in the inability of cells to hydrolyze X-Gal. This results in the production of white colonies amongst a background of blue colonies. This experiment provides a DNA fragment together with a linear plasmid and T4 DNA Ligase. Following the ligation to synthesize the recombinant plasmid, competent E. coli cells are transformed and the number of recombinant antibiotic resistant white and blue colonies are counted. ï¿½-galactosidase activity is assayed from blue and white bacterial cells. Kit includes: instructions, Linearized pUC plasmid and DNA fragment, T4 Ligase, Bacterial LyphoCells for transformation, reconstitution buffer, XGal in solvent, IPTG, calcium chloride, antibiotic, ReadyPour luria broth agar, Luria broth media for recovery, growth media, assay components and plastic supplies. You need: incubation oven, two waterbaths, shaking incubator or shaking waterbath, microwave or hot plate, automatic micropipet and tips, spectrophotometer, balance, centrifuge, microcentrifuge, glassware and cuvettes, distilled water, ice.
Country USA
Brand Edvotek Inc
Manufacturer Edvotek
Binding Misc.
ItemPartNumber 300 EDVA7
Size For 5 Lab Groups
UnitCount 1
ReleaseDate 0000-00-00

Country	USA
Brand	Edvotek Inc
Manufacturer	Edvotek
Binding	Misc.
ItemPartNumber	300 EDVA7
Size	For 5 Lab Groups
UnitCount	1
ReleaseDate	0000-00-00

Edvotek 300 Blue/White Cloning of a DNA Fragment and Assay of -galactosidase, For 5 Lab Groups

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